Enhanced human periodontal ligament stem cell viability and osteogenic differentiation on two implant materials: An experimental in vitro study

Background: Surface roughness of dental implants impacts the survival adult periodontal stem cells and rate differentiation. This research was conducted to test how human ligament behaved on yttria stabilized tetragonal zirconia polycrystals polyetheretherketone (PEEK) discs with different surface topographies. Methods: Discs roughening prepared by sandblasting. Stem were cultivated a polished surface, PEEK sandblasted discs. Cells viability assessed after 24, 48, 72 hours. Scanning electron microscopy used examine adherence attachment cells. Osteoblastic differentiation capacity studied checking mineralization clusters development through alizarin red S staining alkaline phosphatase assay. ANOVA Tukey post hoc for statistical analysis. Results: Polished showed lower cell viability, whereas roughened highest proliferation rates percent. The osteogenic enhanced rough surfaces in comparison surfaces. Sandblasted markedly increased mineralized nodule ALP enzyme activity compared control. Conclusions: Micro- topographies creation implant enhances attachment, differentiation.